Efficient and stable transgene suppression via RNAi in field-grown poplars.
Identifieur interne : 003935 ( Main/Exploration ); précédent : 003934; suivant : 003936Efficient and stable transgene suppression via RNAi in field-grown poplars.
Auteurs : Jingyi Li [États-Unis] ; Amy M. Brunner ; Olga Shevchenko ; Richard Meilan ; Cathleen Ma ; Jeffrey S. Skinner ; Steven H. StraussSource :
- Transgenic research [ 0962-8819 ] ; 2008.
Descripteurs français
- KwdFr :
- ADN des plantes (génétique), ARN des plantes (génétique), Acetyltransferases (physiologie), Données de séquences moléculaires (MeSH), Dosage génique (MeSH), Extinction de l'expression des gènes (MeSH), Interférence par ARN (MeSH), Méthylation de l'ADN (MeSH), Petit ARN interférent (génétique), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), RT-PCR (MeSH), Région 5' flanquante (MeSH), Régions d'ancrage à la matrice nucléaire (génétique), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes végétaux (MeSH), Similitude de séquences d'acides nucléiques (MeSH), Suppression génétique (MeSH), Séquence nucléotidique (MeSH), Transformation génétique (MeSH), Transgènes (physiologie), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- croissance et développement : Populus, Végétaux génétiquement modifiés.
- génétique : ADN des plantes, ARN des plantes, Petit ARN interférent, Populus, Régions d'ancrage à la matrice nucléaire, Régions promotrices (génétique), Végétaux génétiquement modifiés.
- métabolisme : Populus, Végétaux génétiquement modifiés.
- physiologie : Acetyltransferases, Transgènes.
- Données de séquences moléculaires, Dosage génique, Extinction de l'expression des gènes, Interférence par ARN, Méthylation de l'ADN, RT-PCR, Région 5' flanquante, Régulation de l'expression des gènes végétaux, Similitude de séquences d'acides nucléiques, Suppression génétique, Séquence nucléotidique, Transformation génétique.
English descriptors
- KwdEn :
- 5' Flanking Region (MeSH), Acetyltransferases (physiology), Base Sequence (MeSH), DNA Methylation (MeSH), DNA, Plant (genetics), Gene Dosage (MeSH), Gene Expression Regulation, Plant (MeSH), Gene Silencing (MeSH), Matrix Attachment Regions (genetics), Molecular Sequence Data (MeSH), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (growth & development), Populus (metabolism), Promoter Regions, Genetic (genetics), RNA Interference (MeSH), RNA, Plant (genetics), RNA, Small Interfering (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Homology, Nucleic Acid (MeSH), Suppression, Genetic (MeSH), Transformation, Genetic (MeSH), Transgenes (physiology).
- MESH :
- chemical , genetics : DNA, Plant, RNA, Plant, RNA, Small Interfering.
- chemical , physiology : Acetyltransferases.
- genetics : Matrix Attachment Regions, Plants, Genetically Modified, Populus, Promoter Regions, Genetic.
- growth & development : Plants, Genetically Modified, Populus.
- metabolism : Plants, Genetically Modified, Populus.
- physiology : Transgenes.
- 5' Flanking Region, Base Sequence, DNA Methylation, Gene Dosage, Gene Expression Regulation, Plant, Gene Silencing, Molecular Sequence Data, RNA Interference, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Suppression, Genetic, Transformation, Genetic.
Abstract
The efficiency and stability of RNA interference (RNAi) in perennial species, particularly in natural environments, is poorly understood. We studied 56 independent poplar RNAi transgenic events in the field over 2 years. A resident BAR transgene was targeted with two different types of RNAi constructs: a 475-bp IR of the promoter sequence and a 275-bp IR of the coding sequence, each with and without the presence of flanking matrix attachment regions (MARs). RNAi directed at the coding sequence was a strong inducer of gene silencing; 80% of the transgenic events showed more than 90% suppression. In contrast, RNAi targeting the promoter resulted in only 6% of transgenic events showing more than 90% suppression. The degree of suppression varied widely but was highly stable in each event over 2 years in the field, and had no association with insert copy number or the presence of MARs. RNAi remained stable during a winter to summer seasonal cycle, a time when expression of the targeted transgene driven by an rbcS promoter varied widely. When strong gene suppression was induced by an IR directed at the promoter sequence, it was accompanied by methylation of the homologous promoter region. DNA methylation was also observed in the coding region of highly suppressed events containing an IR directed at the coding sequence; however, the methylation degree and pattern varied widely among those suppressed events. Our results suggest that RNAi can be highly effective for functional genomics and biotechnology of perennial plants.
DOI: 10.1007/s11248-007-9148-1
PubMed: 17929189
Affiliations:
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Brunner</name></author><author><name sortKey="Shevchenko, Olga" sort="Shevchenko, Olga" uniqKey="Shevchenko O" first="Olga" last="Shevchenko">Olga Shevchenko</name></author><author><name sortKey="Meilan, Richard" sort="Meilan, Richard" uniqKey="Meilan R" first="Richard" last="Meilan">Richard Meilan</name></author><author><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name></author><author><name sortKey="Skinner, Jeffrey S" sort="Skinner, Jeffrey S" uniqKey="Skinner J" first="Jeffrey S" last="Skinner">Jeffrey S. Skinner</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:17929189</idno><idno type="pmid">17929189</idno><idno type="doi">10.1007/s11248-007-9148-1</idno><idno type="wicri:Area/Main/Corpus">003A52</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003A52</idno><idno type="wicri:Area/Main/Curation">003A52</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003A52</idno><idno type="wicri:Area/Main/Exploration">003A52</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Efficient and stable transgene suppression via RNAi in field-grown poplars.</title><author><name sortKey="Li, Jingyi" sort="Li, Jingyi" uniqKey="Li J" first="Jingyi" last="Li">Jingyi Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-5752, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Science, Oregon State University, Corvallis, OR 97331-5752</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Brunner, Amy M" sort="Brunner, Amy M" uniqKey="Brunner A" first="Amy M" last="Brunner">Amy M. Brunner</name></author><author><name sortKey="Shevchenko, Olga" sort="Shevchenko, Olga" uniqKey="Shevchenko O" first="Olga" last="Shevchenko">Olga Shevchenko</name></author><author><name sortKey="Meilan, Richard" sort="Meilan, Richard" uniqKey="Meilan R" first="Richard" last="Meilan">Richard Meilan</name></author><author><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name></author><author><name sortKey="Skinner, Jeffrey S" sort="Skinner, Jeffrey S" uniqKey="Skinner J" first="Jeffrey S" last="Skinner">Jeffrey S. Skinner</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></analytic><series><title level="j">Transgenic research</title><idno type="ISSN">0962-8819</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>5' Flanking Region (MeSH)</term><term>Acetyltransferases (physiology)</term><term>Base Sequence (MeSH)</term><term>DNA Methylation (MeSH)</term><term>DNA, Plant (genetics)</term><term>Gene Dosage (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Gene Silencing (MeSH)</term><term>Matrix Attachment Regions (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term><term>Promoter Regions, Genetic (genetics)</term><term>RNA Interference (MeSH)</term><term>RNA, Plant (genetics)</term><term>RNA, Small Interfering (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term><term>Suppression, Genetic (MeSH)</term><term>Transformation, Genetic (MeSH)</term><term>Transgenes (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term><term>ARN des plantes (génétique)</term><term>Acetyltransferases (physiologie)</term><term>Données de séquences moléculaires (MeSH)</term><term>Dosage génique (MeSH)</term><term>Extinction de l'expression des gènes (MeSH)</term><term>Interférence par ARN (MeSH)</term><term>Méthylation de l'ADN (MeSH)</term><term>Petit ARN interférent (génétique)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>RT-PCR (MeSH)</term><term>Région 5' flanquante (MeSH)</term><term>Régions d'ancrage à la matrice nucléaire (génétique)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Similitude de séquences d'acides nucléiques (MeSH)</term><term>Suppression génétique (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Transgènes (physiologie)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term><term>RNA, Plant</term><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Acetyltransferases</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Matrix Attachment Regions</term><term>Plants, Genetically Modified</term><term>Populus</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term><term>ARN des plantes</term><term>Petit ARN interférent</term><term>Populus</term><term>Régions d'ancrage à la matrice nucléaire</term><term>Régions promotrices (génétique)</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Acetyltransferases</term><term>Transgènes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>5' Flanking Region</term><term>Base Sequence</term><term>DNA Methylation</term><term>Gene Dosage</term><term>Gene Expression Regulation, Plant</term><term>Gene Silencing</term><term>Molecular Sequence Data</term><term>RNA Interference</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Homology, Nucleic Acid</term><term>Suppression, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Dosage génique</term><term>Extinction de l'expression des gènes</term><term>Interférence par ARN</term><term>Méthylation de l'ADN</term><term>RT-PCR</term><term>Région 5' flanquante</term><term>Régulation de l'expression des gènes végétaux</term><term>Similitude de séquences d'acides nucléiques</term><term>Suppression génétique</term><term>Séquence nucléotidique</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The efficiency and stability of RNA interference (RNAi) in perennial species, particularly in natural environments, is poorly understood. We studied 56 independent poplar RNAi transgenic events in the field over 2 years. A resident BAR transgene was targeted with two different types of RNAi constructs: a 475-bp IR of the promoter sequence and a 275-bp IR of the coding sequence, each with and without the presence of flanking matrix attachment regions (MARs). RNAi directed at the coding sequence was a strong inducer of gene silencing; 80% of the transgenic events showed more than 90% suppression. In contrast, RNAi targeting the promoter resulted in only 6% of transgenic events showing more than 90% suppression. The degree of suppression varied widely but was highly stable in each event over 2 years in the field, and had no association with insert copy number or the presence of MARs. RNAi remained stable during a winter to summer seasonal cycle, a time when expression of the targeted transgene driven by an rbcS promoter varied widely. When strong gene suppression was induced by an IR directed at the promoter sequence, it was accompanied by methylation of the homologous promoter region. DNA methylation was also observed in the coding region of highly suppressed events containing an IR directed at the coding sequence; however, the methylation degree and pattern varied widely among those suppressed events. Our results suggest that RNAi can be highly effective for functional genomics and biotechnology of perennial plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17929189</PMID><DateCompleted><Year>2008</Year><Month>10</Month><Day>09</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0962-8819</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>4</Issue><PubDate><Year>2008</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Transgenic research</Title><ISOAbbreviation>Transgenic Res</ISOAbbreviation></Journal><ArticleTitle>Efficient and stable transgene suppression via RNAi in field-grown poplars.</ArticleTitle><Pagination><MedlinePgn>679-94</MedlinePgn></Pagination><Abstract><AbstractText>The efficiency and stability of RNA interference (RNAi) in perennial species, particularly in natural environments, is poorly understood. We studied 56 independent poplar RNAi transgenic events in the field over 2 years. A resident BAR transgene was targeted with two different types of RNAi constructs: a 475-bp IR of the promoter sequence and a 275-bp IR of the coding sequence, each with and without the presence of flanking matrix attachment regions (MARs). RNAi directed at the coding sequence was a strong inducer of gene silencing; 80% of the transgenic events showed more than 90% suppression. In contrast, RNAi targeting the promoter resulted in only 6% of transgenic events showing more than 90% suppression. The degree of suppression varied widely but was highly stable in each event over 2 years in the field, and had no association with insert copy number or the presence of MARs. RNAi remained stable during a winter to summer seasonal cycle, a time when expression of the targeted transgene driven by an rbcS promoter varied widely. When strong gene suppression was induced by an IR directed at the promoter sequence, it was accompanied by methylation of the homologous promoter region. DNA methylation was also observed in the coding region of highly suppressed events containing an IR directed at the coding sequence; however, the methylation degree and pattern varied widely among those suppressed events. Our results suggest that RNAi can be highly effective for functional genomics and biotechnology of perennial plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jingyi</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-5752, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brunner</LastName><ForeName>Amy M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Shevchenko</LastName><ForeName>Olga</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Meilan</LastName><ForeName>Richard</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Cathleen</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Skinner</LastName><ForeName>Jeffrey S</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven H</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>10</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Transgenic Res</MedlineTA><NlmUniqueID>9209120</NlmUniqueID><ISSNLinking>0962-8819</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D034741">RNA, Small Interfering</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.3.1.-</RegistryNumber><NameOfSubstance UI="D000123">Acetyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.3.1.-</RegistryNumber><NameOfSubstance UI="C056243">phosphinothricin N-acetyltransferase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D024506" MajorTopicYN="N">5' Flanking Region</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000123" MajorTopicYN="N">Acetyltransferases</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019175" MajorTopicYN="N">DNA Methylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018628" MajorTopicYN="N">Gene Dosage</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="Y">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045170" MajorTopicYN="N">Matrix Attachment Regions</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="N">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034741" MajorTopicYN="N">RNA, Small Interfering</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013489" MajorTopicYN="Y">Suppression, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2007</Year><Month>06</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2007</Year><Month>09</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>10</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>10</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>10</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17929189</ArticleId><ArticleId IdType="doi">10.1007/s11248-007-9148-1</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Cell Rep. 2006 Jul;25(7):660-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16496153</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2007 Apr;143(4):1441-51</Citation><ArticleIdList><ArticleId IdType="pubmed">17307899</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2002 Nov;18(11):1427-31</Citation><ArticleIdList><ArticleId 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IdType="pubmed">12953121</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><noCountry><name sortKey="Brunner, Amy M" sort="Brunner, Amy M" uniqKey="Brunner A" first="Amy M" last="Brunner">Amy M. Brunner</name><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name><name sortKey="Meilan, Richard" sort="Meilan, Richard" uniqKey="Meilan R" first="Richard" last="Meilan">Richard Meilan</name><name sortKey="Shevchenko, Olga" sort="Shevchenko, Olga" uniqKey="Shevchenko O" first="Olga" last="Shevchenko">Olga Shevchenko</name><name sortKey="Skinner, Jeffrey S" sort="Skinner, Jeffrey S" uniqKey="Skinner J" first="Jeffrey S" last="Skinner">Jeffrey S. Skinner</name><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></noCountry><country name="États-Unis"><region name="Oregon"><name sortKey="Li, Jingyi" sort="Li, Jingyi" uniqKey="Li J" first="Jingyi" last="Li">Jingyi Li</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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